Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens

Sucheeva JUNNU; Preeda LERTWATCHARASARAKUL; Siriluk JALA; Sakuna PHATTANAKUNANAN; Pattra MOONJIT; Thaweesak SONGSERM

doi:10.1292/jvms.13-0196

Virology

Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens

Sucheeva JUNNU, Preeda LERTWATCHARASARAKUL, Siriluk JALA, Sakuna PHATTANAKUNANAN, Pattra MOONJIT, Thaweesak SONGSERM

Author information

Sucheeva JUNNU
Center for Agricultural Biotechnology, Kasetsart University, Nakhon Pathom 73140, Thailand
Center of Excellence on Agricultural Biotechnology, Science and Technology Postgraduate Education and Research Development Office, Commission on Higher Education, Ministry of Education (AG-BIO/PERDO-CHE), Thailand
Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand
Preeda LERTWATCHARASARAKUL
Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
Siriluk JALA
Center of Veterinary Research and Service, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
Sakuna PHATTANAKUNANAN
Center of Veterinary Research and Service, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
Pattra MOONJIT
Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
Thaweesak SONGSERM
Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand

Keywords: chickens, enzyme-linked immunosorbent assay (ELISA), fowl adenovirus, inclusion body hepatitis, recombinant hexon protein

JOURNAL FREE ACCESS

2014 Volume 76 Issue 2 Pages 289-293

DOI https://doi.org/10.1292/jvms.13-0196

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Abstract

Fowl adenovirus (FAdv) serotype 2 causes inclusion body hepatitis (IBH) disease which adversely affects the broiler industry in Thailand. We developed an indirect ELISA based on the recombinant hexon protein produced by E. coli. The recombinant hexon protein was tested with sera, in both infected and noninfected chickens. The recombinant hexon protein was standardized with an antigen concentration of 3.75 µg/ml and test sera. The intra- and inter-assays were repeatable. The cutoff value from TG-ROC curve analysis was 0.106. The specificity and sensitivity were 80 and 80%, respectively. The correlation coefficient (r) of absorbance values from this ELISA compared with the serum neutralization test was 0.76. This ELISA might be helpful for IBH diagnosis and surveillance.

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