Journal of Veterinary Medical Science
Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250

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Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens
Sucheeva JUNNUPreeda LERTWATCHARASARAKULSiriluk JALASakuna PHATTANAKUNANANPattra MOONJITThaweesak SONGSERM
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JOURNAL FREE ACCESS Advance online publication

Article ID: 13-0196

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Abstract

Fowl Adenovirus (FAdv) serotype 2 causes inclusion body hepatitis (IBH) disease which adversely affects the broiler industry in Thailand. We developed an indirect ELISA based on the recombinant hexon protein produced by E.coli. The recombinant hexon protein was tested with sera, in both infected and non-infected chickens. The recombinant hexon protein was standardized with an antigen concentration of 3.75 µg/ml and test sera. The intra and inter assay were repeatable. The cut-off value from TG-ROC curve analysis was 0.106. The specificity and sensitivity were 80 and 80%, respectively. The correlation coefficient (r) of absorbance values from ELISA compared with serum neutralization test was 0.76. The ELISA might be helpful for IBH diagnosis and surveillance.

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© 2013 by the Japanese Society of Veterinary Science

この記事はクリエイティブ・コモンズ [表示 - 非営利 - 改変禁止 4.0 国際]ライセンスの下に提供されています。
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.ja
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