Article ID: 13-0196
Fowl Adenovirus (FAdv) serotype 2 causes inclusion body hepatitis (IBH) disease which adversely affects the broiler industry in Thailand. We developed an indirect ELISA based on the recombinant hexon protein produced by E.coli. The recombinant hexon protein was tested with sera, in both infected and non-infected chickens. The recombinant hexon protein was standardized with an antigen concentration of 3.75 µg/ml and test sera. The intra and inter assay were repeatable. The cut-off value from TG-ROC curve analysis was 0.106. The specificity and sensitivity were 80 and 80%, respectively. The correlation coefficient (r) of absorbance values from ELISA compared with serum neutralization test was 0.76. The ELISA might be helpful for IBH diagnosis and surveillance.