Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens

Sucheeva JUNNU; Preeda LERTWATCHARASARAKUL; Siriluk JALA; Sakuna PHATTANAKUNANAN; Pattra MOONJIT; Thaweesak SONGSERM

doi:10.1292/jvms.13-0196

This article has now been updated. Please use the final version.

Developing an Indirect ELISA Based on Recombinant Hexon Protein for Serological Detection of Inclusion Body Hepatitis in Chickens

Sucheeva JUNNU, Preeda LERTWATCHARASARAKUL, Siriluk JALA, Sakuna PHATTANAKUNANAN, Pattra MOONJIT, Thaweesak SONGSERM

Author information

Sucheeva JUNNU
Center for Agricultural Biotechnology, Kasetsart University
Center of Excellence on Agricultural Biotechnology, Science and Technology Postgraduate Education and Research Development Office, Commission on Higher Education, Ministry of Education
Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University
Preeda LERTWATCHARASARAKUL
Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University
Siriluk JALA
Center of Veterinary Research and Service, Faculty of Veterinary Medicine, Kasetsart University
Sakuna PHATTANAKUNANAN
Center of Veterinary Research and Service, Faculty of Veterinary Medicine, Kasetsart University
Pattra MOONJIT
Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine, Kasetsart University
Thaweesak SONGSERM
Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University
Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University

Keywords: chickens, enzyme linked immuno-sorbent assay (ELISA), fowl adenovirus, inclusion body hepatitis, recombinant hexon protein

JOURNAL FREE ACCESS Advance online publication

Article ID: 13-0196

DOI https://doi.org/10.1292/jvms.13-0196

The final version of this article is now available: Vol. 76 (2014), No. 2 pp. 289-293

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Abstract

Fowl Adenovirus (FAdv) serotype 2 causes inclusion body hepatitis (IBH) disease which adversely affects the broiler industry in Thailand. We developed an indirect ELISA based on the recombinant hexon protein produced by E.coli. The recombinant hexon protein was tested with sera, in both infected and non-infected chickens. The recombinant hexon protein was standardized with an antigen concentration of 3.75 µg/ml and test sera. The intra and inter assay were repeatable. The cut-off value from TG-ROC curve analysis was 0.106. The specificity and sensitivity were 80 and 80%, respectively. The correlation coefficient (r) of absorbance values from ELISA compared with serum neutralization test was 0.76. The ELISA might be helpful for IBH diagnosis and surveillance.

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