Detection of Japanese eel endothelial cells-infecting virus in Anguilla japonica elvers

Sachiko OKAZAKI; Shinya YASUMOTO; Satoshi KOYAMA; Shinobu TSUCHIAKA; Yuki NAOI; Tsutomu OMATSU; Shin-ichi ONO; Tetsuya MIZUTANI

doi:10.1292/jvms.15-0515

Wildlife Science

Detection of Japanese eel endothelial cells-infecting virus in Anguilla japonica elvers

Sachiko OKAZAKI, Shinya YASUMOTO, Satoshi KOYAMA, Shinobu TSUCHIAKA, Yuki NAOI, Tsutomu OMATSU, Shin-ichi ONO, Tetsuya MIZUTANI

著者情報

Sachiko OKAZAKI
The United Graduate School of Veterinary Sciences, Gifu University, 1–1 Yanagito, Gifu-shi, Gifu 501–1193, Japan
Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3–5–8 Saiwai-cho, Fuchu, Tokyo 183–0054, Japan
Shinya YASUMOTO
Department of Applied Aquabiology, National Fisheries University, 2–7–1, Nagata-Honmachi, Shimonoseki, Yamaguchi 759–6595, Japan
Satoshi KOYAMA
The United Graduate School of Veterinary Sciences, Gifu University, 1–1 Yanagito, Gifu-shi, Gifu 501–1193, Japan
Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3–5–8 Saiwai-cho, Fuchu, Tokyo 183–0054, Japan
Shinobu TSUCHIAKA
The United Graduate School of Veterinary Sciences, Gifu University, 1–1 Yanagito, Gifu-shi, Gifu 501–1193, Japan
Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3–5–8 Saiwai-cho, Fuchu, Tokyo 183–0054, Japan
Yuki NAOI
Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3–5–8 Saiwai-cho, Fuchu, Tokyo 183–0054, Japan
Tsutomu OMATSU
The United Graduate School of Veterinary Sciences, Gifu University, 1–1 Yanagito, Gifu-shi, Gifu 501–1193, Japan
Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3–5–8 Saiwai-cho, Fuchu, Tokyo 183–0054, Japan
Shin-ichi ONO
School of Marine Science and Technology, Tokai University, 3–20–1 Orido, Shimizu-ku, Shizuoka 424–0902, Japan
Tetsuya MIZUTANI
The United Graduate School of Veterinary Sciences, Gifu University, 1–1 Yanagito, Gifu-shi, Gifu 501–1193, Japan
Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3–5–8 Saiwai-cho, Fuchu, Tokyo 183–0054, Japan

キーワード: Anguilla japonica, elver, Japanese eel endothelial cells-infecting virus

ジャーナルフリー

2016 年 78 巻 4 号 p. 705-707

DOI https://doi.org/10.1292/jvms.15-0515

Browse “Advance online publication” version

詳細

抄録

Japanese eel endothelial cells-infecting virus (JEECV) has spread in eel farms and caused serious economic loss. In this study, we examined the prevalence of JEECV infection in 100 wild Japanese eel (Anguilla japonica) elvers caught from Yamaguchi prefecture, Japan, using quantitative PCR and conventional PCR. Total genomic DNA was obtained from the cranial quarter of the body in 70 of 100 eels and from the gill in the remaining. Of 30 gill samples, 20 were analyzed after pooling with other samples, and the remaining 10 were analyzed separately. A single positive result for JEECV was detected following analysis of the 10 separately analyzed samples. This result constitutes the first report of JEECV infection in wild A. japonica elvers.

責任著者(Corresponding author)

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