Persistence of chicken anemia virus antigen and inclusions in spontaneous          cases of Marek’s disease visceral lymphomas in broiler chickens at          slaughterhouses

Mohamed Sabry AHMED; Hiroki ONO; Jun SASAKI; Kenji OCHIAI; Masanobu GORYO

doi:10.1292/jvms.15-0615

Avian Pathology

Persistence of chicken anemia virus antigen and inclusions in spontaneous cases of Marek’s disease visceral lymphomas in broiler chickens at slaughterhouses

Mohamed Sabry AHMED, Hiroki ONO, Jun SASAKI, Kenji OCHIAI, Masanobu GORYO

Author information

Mohamed Sabry AHMED
Department of Pathogenic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Iwate 020–8550, Japan
Department of Poultry Diseases, Faculty of Veterinary Medicine, South Valley University, Qena 83523, Egypt
Hiroki ONO
Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Iwate 020–8550, Japan
Jun SASAKI
Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Iwate 020–8550, Japan
Kenji OCHIAI
Department of Pathogenic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Iwate 020–8550, Japan
Masanobu GORYO
Department of Pathogenic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Iwate 020–8550, Japan

Keywords: chicken anemia virus, co-infection, inclusion body, lymphoma, marek’s disease

JOURNAL FREE ACCESS

2016 Volume 78 Issue 5 Pages 825-829

DOI https://doi.org/10.1292/jvms.15-0615

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Abstract

The chicken anemia virus (CAV) and Marek’s disease virus (MDV) infect chickens worldwide; a single or dual infection by these viruses has a great impact on poultry production. In the present study, we examined the existence of CAV antigen and its inclusions in Marek’s disease (MD) lymphomas in chickens in the slaughterhouses of Iwate prefecture, Japan. Forty-nine spleens and 13 livers with different degrees of nodular lesions were histopathologically examined at our laboratory. Grossly, the tested organs showed various sizes and anatomical architectures. Based on the cellular morphology and the infiltrative nature of the neoplastic lymphocytes, MD was confirmed in 76% (37/49) of the spleens and 92% (12/13) of the livers. The lesions of MD, according to the pattern of lymphocytic accumulation in the affected organs, were divided into multifocal, coalesced and diffuse. CAV intranuclear inclusion bodies were detected within the small and the large bizarre lymphocytes of the MD lymphomas in 2 livers and 9 spleens, and the immunostaining test for CAV confirmed the persistence of CAV antigens and inclusions in the neoplastic cells. This study demonstrated the persistence of CAV infection within the neoplastic cells of naturally occurring MD lymphomas in chickens.

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