Calcinated egg shell as a candidate of biosecurity enhancement          material

Mari OTA; Chiharu TOYOFUKU; Chanathip THAMMAKARN; Natthanan SANGSRIRATANAKUL; Masashi YAMADA; Katsuhiro NAKAJIMA; Minori KITAZAWA; Hakimullah HAKIM; Md. Shahin ALAM; Dany SHOHAM; Kazuaki TAKEHARA

doi:10.1292/jvms.16-0004

Avian Pathology

Calcinated egg shell as a candidate of biosecurity enhancement material

Mari OTA, Chiharu TOYOFUKU, Chanathip THAMMAKARN, Natthanan SANGSRIRATANAKUL, Masashi YAMADA, Katsuhiro NAKAJIMA, Minori KITAZAWA, Hakimullah HAKIM, Md. Shahin ALAM, Dany SHOHAM, Kazuaki TAKEHARA

Author information

Mari OTA
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3–5–8, Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
Chiharu TOYOFUKU
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3–5–8, Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
Chanathip THAMMAKARN
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3–5–8, Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
The United Graduate School of Veterinary Science, Gifu University, 1–1, Yanagido, Gifu 501–1193, Japan
Natthanan SANGSRIRATANAKUL
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3–5–8, Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
The United Graduate School of Veterinary Science, Gifu University, 1–1, Yanagido, Gifu 501–1193, Japan
Masashi YAMADA
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3–5–8, Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
Katsuhiro NAKAJIMA
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3–5–8, Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
Minori KITAZAWA
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3–5–8, Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
Hakimullah HAKIM
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3–5–8, Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
The United Graduate School of Veterinary Science, Gifu University, 1–1, Yanagido, Gifu 501–1193, Japan
Md. Shahin ALAM
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3–5–8, Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
The United Graduate School of Veterinary Science, Gifu University, 1–1, Yanagido, Gifu 501–1193, Japan
Dany SHOHAM
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3–5–8, Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
Kazuaki TAKEHARA
Laboratory of Animal Health, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3–5–8, Saiwai-cho, Fuchu-shi, Tokyo 183–8509, Japan
The United Graduate School of Veterinary Science, Gifu University, 1–1, Yanagido, Gifu 501–1193, Japan

Keywords: calcinated egg shell (Egg-CaO), HACCP at farm level, inactivation of avian pathogen, livestock biosecurity

JOURNAL FREE ACCESS

2016 Volume 78 Issue 5 Pages 831-836

DOI https://doi.org/10.1292/jvms.16-0004

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Abstract

Calcinated egg shell (Egg-CaO), of which the main component is calcium oxide, was evaluated in the forms of powder and aqueous solutions for their efficacies as disinfectants against avian influenza virus (AIV), Newcastle disease virus (NDV), infectious bursal disease virus (IBDV), Salmonella Infantis and Escherichia coli. Egg-CaO powder inactivated these viruses within 3 min in the presence of 33% of fetal bovine serum (FBS). In Egg-CaO solutions, except AIV, all pathogens were inactivated within 1 hr, even in the presence of 5% of FBS. Without FBS, all pathogens, except AIV, were inactivated within 3 min, and AIV within 1 hr. In addition, persistence of virucidal activity against AIV and NDV of Egg-CaO powder was confirmed after exposure to sunlight for 2 weeks or resuspension with water for 7 times, simulating field harsh environments. Chick growth test was conducted to ensure the safety of the use of Egg-CaO powder in chicken cages and showed that it is safe to add Egg-CaO in litter or feed. In conclusion, Egg-CaO can be useful for the enhancement of biosecurity at farms.

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