Comparison of two agar gel immunodiffusion protocols for diagnosing equine          infectious anemia

Manabu NEMOTO; Takashi YAMANAKA; Hiroshi BANNAI; Koji TSUJIMURA; Takanori UENO; Hirohisa MEKATA; Ayako YOSHIDA; Aki KOYAMA; Hiroshi KOKADO

doi:10.1292/jvms.18-0103

Virology

Comparison of two agar gel immunodiffusion protocols for diagnosing equine infectious anemia

Manabu NEMOTO, Takashi YAMANAKA, Hiroshi BANNAI, Koji TSUJIMURA, Takanori UENO, Hirohisa MEKATA, Ayako YOSHIDA, Aki KOYAMA, Hiroshi KOKADO

著者情報

Manabu NEMOTO
Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan
Takashi YAMANAKA
Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan
Hiroshi BANNAI
Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan
Koji TSUJIMURA
Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan
Takanori UENO
Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan
Hirohisa MEKATA
Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan
Ayako YOSHIDA
Center for Animal Disease Control, University of Miyazaki, 1-1 Gakuen-Kibanadai-Nishi, Miyazaki 889-2192, Japan
Aki KOYAMA
IDEXX Laboratories K.K., 1-22-19 Izumi, Suginami-ku, Tokyo 168-0063, Japan
Hiroshi KOKADO
Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotsuke, Tochigi 329-0412, Japan

キーワード: agar gel immunodiffusion, equine infectious anemia, Japanese act, OIE

ジャーナルフリー

2018 年 80 巻 8 号 p. 1245-1247

DOI https://doi.org/10.1292/jvms.18-0103

Browse “Advance online publication” version

詳細

抄録

This study compared agar gel immunodiffusion (AGID) protocols for diagnosing equine infectious anemia. Two commercial testing kits were used: one following the Japanese Act on Domestic Animal Infectious Diseases Control and one following the World Organisation for Animal Health (OIE) manual. From 651 samples tested, both protocols gave identical results for 647 samples (23 samples tested positive; 624 tested negative). Non-specific reactions were observed in 21 samples testing negative by the Japanese protocol, but none were observed with the OIE protocol. The kappa coefficient value was 0.962, indicating almost perfect agreement between the two protocols. This study found no difference in diagnostic agreement between the two protocols, but the OIE protocol produced non-specific reactions less frequently than the Japanese protocol.

責任著者(Corresponding author)

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