One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus

Dafei LIU; Fei LIU; Dongchun GUO; Xiaoliang HU; Zhijie LI; Zhigang LI; Jianzhang MA; Chunguo LIU

doi:10.1292/jvms.17-0442

Virology

One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus

Dafei LIU, Fei LIU, Dongchun GUO, Xiaoliang HU, Zhijie LI, Zhigang LI, Jianzhang MA, Chunguo LIU

Author information

Dafei LIU
College of Wildlife Resources, Northeast Forestry University, Harbin, Heilongjiang 150040, China
State Key Lab of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150069, China
Fei LIU
Shanghai Hile Bio–Pharmaceutical Co., Ltd., Shanghai, 201403, China
Dongchun GUO
State Key Lab of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150069, China
Xiaoliang HU
State Key Lab of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150069, China
Zhijie LI
State Key Lab of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150069, China
Zhigang LI
Wendengying Veterinary Station, Weihai, Shandong 264413, China
Jianzhang MA
College of Wildlife Resources, Northeast Forestry University, Harbin, Heilongjiang 150040, China
Chunguo LIU
State Key Lab of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang 150069, China

Keywords: canine distemper virus (CDV), canine kobuvirus (CaKoV), canine parvovirus (CPV), one-step triplex PCR/RT-PCR

JOURNAL FREE ACCESS

2019 Volume 81 Issue 7 Pages 1040-1042

DOI https://doi.org/10.1292/jvms.17-0442

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Abstract

To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 10^2.1 TCID₅₀ for CDV, 10^1.9 TCID₅₀ for CPV and 10³ copies for CaKoV. This method did not amplify nonspecific DNA or RNA from other canine viruses. Therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of CDV, CPV and CaKoV in dogs.

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