Identification of bovine papillomavirus type 1 and 2 from bovine anogenital fibropapillomas

Nanako YAMASHITA-KAWANISHI; Masano TSUZUKI; Zhu WEI; Mun Keong KOK; Dai ISHIYAMA; James K. CHAMBERS; Kazuyuki UCHIDA; Jianbao DONG; Hidekatsu SHIMAKURA; Takeshi HAGA

doi:10.1292/jvms.19-0017

Clinical Pathology

Identification of bovine papillomavirus type 1 and 2 from bovine anogenital fibropapillomas

Nanako YAMASHITA-KAWANISHI, Masano TSUZUKI, Zhu WEI, Mun Keong KOK, Dai ISHIYAMA, James K. CHAMBERS, Kazuyuki UCHIDA, Jianbao DONG, Hidekatsu SHIMAKURA, Takeshi HAGA

Author information

Nanako YAMASHITA-KAWANISHI
Division of Infection Control and Disease Prevention, Department of Veterinary Medical Science, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Masano TSUZUKI
Division of Infection Control and Disease Prevention, Department of Veterinary Medical Science, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Zhu WEI
Division of Infection Control and Disease Prevention, Department of Veterinary Medical Science, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Shandong Vocational Animal Science and Veterinary College, 88, Shenglidong Street, Weifang 261061, China
The Co-constructing State Key Laboratory of Three Rivers Sources Ecology and Plateau Agriculture and Animal Husbandry, Qinghai University, 25 Ningda Road, Xining 810016, China
Mun Keong KOK
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Dai ISHIYAMA
Yachiyo Branch Office, Western Veterinary Clinical Center, Chiba Prefectural Federated Agricultural Mutual Aid Association, 2-5-12 Midorigaoka, Yachiyo-shi, Chiba 276-0049, Japan
James K. CHAMBERS
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Kazuyuki UCHIDA
Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Jianbao DONG
Division of Infection Control and Disease Prevention, Department of Veterinary Medical Science, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Shandong Vocational Animal Science and Veterinary College, 88, Shenglidong Street, Weifang 261061, China
The Co-constructing State Key Laboratory of Three Rivers Sources Ecology and Plateau Agriculture and Animal Husbandry, Qinghai University, 25 Ningda Road, Xining 810016, China
Hidekatsu SHIMAKURA
Division of Infection Control and Disease Prevention, Department of Veterinary Medical Science, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
Takeshi HAGA
Division of Infection Control and Disease Prevention, Department of Veterinary Medical Science, Graduate School of Agriculture and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan

Keywords: anogenital tumor, bovine papillomavirus, cattle, fibropapilloma

JOURNAL FREE ACCESS

2019 Volume 81 Issue 7 Pages 1000-1005

DOI https://doi.org/10.1292/jvms.19-0017

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Abstract

Papillomavirus (PV) is a well-known pathogen associated with epithelial and mucosal neoplastic diseases. In contrast to human PVs, characterization of animal PVs from the aspect of anogenital neoplasm is still on a learning curve. In the present study, two vulval and one anal warts, histologically diagnosed as fibropapillomas, excised from dairy cattle were analyzed. PCR and sequencing revealed that bovine papillomavirus type 1 (BPV-1) and BPV-2 were detected from anal and vulval fibropapillomas, respectively. Immunohistochemistry detected PV antigen in a few differentiated keratinocytes of one vulval case. Reverse-transcriptase PCR detected the early region, but not the late region of BPV mRNA in all three cases. The present study will provide new insight into the relationship between BPV and anogenital papilloma in cattle.

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