Bovine leukemia virus genotype surveillance in cattle at a slaughterhouse in Aichi Prefecture, Japan, in 2019 using polymerase chain reaction combined with restriction fragment length polymorphism

Yuma SAHASHI; Masaaki OSHIMA; Junjiro YAMAGISHI; Chieko MURAMATSU; Kaori SHIMIZU; Yasuo INOSHIMA

doi:10.1292/jvms.21-0234

Public Health

Bovine leukemia virus genotype surveillance in cattle at a slaughterhouse in Aichi Prefecture, Japan, in 2019 using polymerase chain reaction combined with restriction fragment length polymorphism

Yuma SAHASHI, Masaaki OSHIMA, Junjiro YAMAGISHI, Chieko MURAMATSU, Kaori SHIMIZU, Yasuo INOSHIMA

著者情報

Yuma SAHASHI
Nagoya City Meat Hygiene Inspection Laboratory, 1-39 Funami, Minato, Nagoya, Aichi 455-0027, Japan
Present address: Nagoya City Agricultural Center dela Farm, 2872-3 Aza Kuroishi, Oaza Hirabari, Tenpaku, Nagoya, Aichi 468-0021, Japan
Masaaki OSHIMA
Nagoya City Meat Hygiene Inspection Laboratory, 1-39 Funami, Minato, Nagoya, Aichi 455-0027, Japan
Junjiro YAMAGISHI
Nagoya City Meat Hygiene Inspection Laboratory, 1-39 Funami, Minato, Nagoya, Aichi 455-0027, Japan
Chieko MURAMATSU
Nagoya City Meat Hygiene Inspection Laboratory, 1-39 Funami, Minato, Nagoya, Aichi 455-0027, Japan
Kaori SHIMIZU
Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan
Yasuo INOSHIMA
Laboratory of Food and Environmental Hygiene, Cooperative Department of Veterinary Medicine, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan
Education and Research Center for Food Animal Health, Gifu University (GeFAH), 1-1 Yanagido, Gifu, Gifu 501-1193, Japan
The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan
Joint Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan

キーワード: bovine leukemia virus, epidemiological analysis, genotype, polymerase chain reaction-restriction fragment length polymorphism, sequencing

ジャーナルオープンアクセス

2021 年 83 巻 11 号 p. 1730-1734

DOI https://doi.org/10.1292/jvms.21-0234

Browse “Advance online publication” version

詳細

抄録

Polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP) is commonly used for genotyping bovine leukemia virus (BLV) in slaughterhouses. However, unclassified BLV genotypes have been sporadically reported. To assess the current status of BLV genetic characterization in cattle, PCR-RFLP was performed on blood samples of 170 cattle (84 Japanese Black, 60 Japanese Black x Holstein, and 26 Holstein) from 17 farms (5 prefectures) at a slaughterhouse in Aichi Prefecture in 2019. A total of 65 samples (38.2%) were BLV positive, and genotype 1 was the most predominant (56/65 samples), followed by genotypes 3 (6 samples) and 5 (1 sample), and two unclassified samples. No relationship between the genotypes and breeds was observed. Sequence and phylogenetic analyses demonstrated that unclassified BLV genotypes clustered with genotype 1 sequences were, therefore, not new genotypes.

責任著者(Corresponding author)

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