Bovine leukemia virus genotype surveillance in cattle at a slaughterhouse in Aichi Prefecture, Japan, in 2019 using polymerase chain reaction combined with restriction fragment length polymorphism

Yuma SAHASHI; Masaaki OHSHIMA; Jyunjirou YAMAGISHI; Chieko MURAMATSU; Kaori SHIMIZU; Yasuo INOSHIMA

doi:10.1292/jvms.21-0234

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Bovine leukemia virus genotype surveillance in cattle at a slaughterhouse in Aichi Prefecture, Japan, in 2019 using polymerase chain reaction combined with restriction fragment length polymorphism

Yuma SAHASHI, Masaaki OHSHIMA, Jyunjirou YAMAGISHI, Chieko MURAMATSU, Kaori SHIMIZU, Yasuo INOSHIMA

著者情報

キーワード: bovine leukemia virus, epidemiological analysis, genotype, polymerase chain reaction-restriction fragment length polymorphism, sequencing

ジャーナルオープンアクセス早期公開

論文ID: 21-0234

DOI https://doi.org/10.1292/jvms.21-0234

この記事には本公開記事があります。

The final version of this article is now available: Vol. 83 (2021), No. 11 pp. 1730-1734

詳細

抄録

Polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP) is commonly used for genotyping bovine leukemia virus (BLV) in slaughterhouses. However, unclassified BLV genotypes have been sporadically reported. To assess the current status of BLV genetic characterization in cattle, PCR-RFLP was performed on blood samples of 170 cattle (84 Japanese Black, 60 Japanese Black x Holstein, and 26 Holstein) from 17 farms (5 prefectures) at a slaughterhouse in Aichi Prefecture in 2019. A total of 65 samples (38.2%) were BLV positive, and genotype 1 was the most predominant (56/65 samples), followed by genotypes 3 (6 samples) and 5 (1 sample), and two unclassified samples. No relationship between the genotypes and breeds was observed. Sequence and phylogenetic analyses demonstrated that unclassified BLV genotypes clustered with genotype 1 sequences and were, therefore, not new genotypes.

責任著者(Corresponding author)

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