Cytochrome P450 1A Dependent Activities in Deer, Cattle, and Horses

Wageh Sobhy DARWISH; Yoshinori IKENAKA; Elsaid Abozeid ELDALY; Marumi OHNO; Kentaro Q. SAKAMOTO; Shoichi FUJITA; Mayumi ISHIZUKA

doi:10.1292/jvms.09-0318

This article has now been updated. Please use the final version.

Cytochrome P450 1A Dependent Activities in Deer, Cattle, and Horses

Wageh Sobhy DARWISH, Yoshinori IKENAKA, Elsaid Abozeid ELDALY, Marumi OHNO, Kentaro Q. SAKAMOTO, Shoichi FUJITA, Mayumi ISHIZUKA

Author information

Wageh Sobhy DARWISH
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
Food Control Department, Faculty of Veterinary Medicine, Zagazig University
Yoshinori IKENAKA
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
Elsaid Abozeid ELDALY
Food Control Department, Faculty of Veterinary Medicine, Zagazig University
Marumi OHNO
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
Kentaro Q. SAKAMOTO
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
Shoichi FUJITA
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
Mayumi ISHIZUKA
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University

Keywords: CYP1A1, cytochrome P450, ethoxyresorufin O-deethylase, horse, ungulate

JOURNAL FREE ACCESS Advance online publication

Article ID: 09-0318

DOI https://doi.org/10.1292/jvms.09-0318

The final version of this article is now available: Vol. 72 (2010) , No. 5 p.561

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Abstract

The objective of this study was to investigate and characterize the metabolic activities of CYP1A in deer, cattle, and horses in a comparison to those of rats using ethoxyresorufin O-deethylation (EROD) and methoxyresorufin O-demethylation (MROD) assays. We performed an inhibition study for these activities using anti-rat CYP1A1 antibody, and identified that these activities were due to CYP1A subfamily. Inter-species difference in the CYP1A dependent activities was highly observed in this study. Especially, we found that the horse had the highest EROD and MROD activities among the examined animal species in this study. In the kinetic analysis, horse showed the highest Vmax and catalytic efficiency (Vmax/Km) followed by cattle, deer and finally rats.

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