Cytochrome P450 1A-Dependent Activities in Deer, Cattle and Horses

Wageh Sobhy DARWISH; Yoshinori IKENAKA; Elsaid Abozeid ELDALY; Marumi OHNO; Kentaro Q. SAKAMOTO; Shoichi FUJITA; Mayumi ISHIZUKA

doi:10.1292/jvms.09-0318

Toxicology

Cytochrome P450 1A-Dependent Activities in Deer, Cattle and Horses

Wageh Sobhy DARWISH, Yoshinori IKENAKA, Elsaid Abozeid ELDALY, Marumi OHNO, Kentaro Q. SAKAMOTO, Shoichi FUJITA, Mayumi ISHIZUKA

Author information

Wageh Sobhy DARWISH
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
Food Control Department, Faculty of Veterinary Medicine, Zagazig University
Yoshinori IKENAKA
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
Elsaid Abozeid ELDALY
Food Control Department, Faculty of Veterinary Medicine, Zagazig University
Marumi OHNO
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
Kentaro Q. SAKAMOTO
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
Shoichi FUJITA
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University
Mayumi ISHIZUKA
Laboratory of Toxicology, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University

Keywords: CYP1A1, cytochrome P450, equine, ethoxyresorufin O-deethylase, ungulates

JOURNAL FREE ACCESS

2010 Volume 72 Issue 5 Pages 561-566

DOI https://doi.org/10.1292/jvms.09-0318

View "Advance Publication" version (January 13, 2010).

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Abstract

The objective of this study was to investigate and characterize the metabolic activities of CYP1A in deer, cattle and horses in comparison to those of rats using ethoxyresorufin O-deethylation (EROD) and methoxyresorufin O-demethylation (MROD) assays. We performed an inhibition study for these activities using anti-rat CYP1A1 antibody and identified that these activities were due to the CYP1A subfamily. Interspecies differences in the CYP1A-dependent activities were highly observed in this study. In particular, we found that the horse had the highest EROD and MROD activities among the examined animal species. In the kinetic analysis, the horses showed the highest Vmax and catalytic efficiency (Vmax/Km), followed by the cattle, deer and rats.

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