Molecular Analysis of Cryptosporidium parvum HNJ-1 Isolated in Japan

Said AMER; Ryuma MATSUBARA; Fumi MURAKOSHI; Yutaka NAKAI

doi:10.1292/jvms.10-0118

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Molecular Analysis of Cryptosporidium parvum HNJ-1 Isolated in Japan

Said AMER, Ryuma MATSUBARA, Fumi MURAKOSHI, Yutaka NAKAI

著者情報

キーワード: Cryptosporidium parvum, DHFR, GP60, HNJ-1, ITS

ジャーナルフリー早期公開

論文ID: 10-0118

DOI https://doi.org/10.1292/jvms.10-0118

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2010) , No. 12 p.1647

詳細

抄録

Cryptosporidium parvum HNJ-1 is widely used as reference strain in Japan. In the present study, the parasite was subjected for further molecular analysis including transcribed ribosomal region (ITS rRNA), dihydrofolate reductase (DHFR) and surface glycoprotein (GP60) genes. Partial sequence analysis of these genes indicated extensive polymorphism in ITS region compared with relevant sequences of other Cryptosporidium parvum isolates. In addition, this strain was identified as C. parvum IIaA15G2R1 subtype, based on sequence results of GP60 gene locus.

責任著者(Corresponding author)

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