Abstract
The study described the construction and transfection of EGFP-fused Porcine Circovirus Type 2 (PCV2) genome and the recovery of the virus. Post-transfection, PCV2 (ORF1)-EGFP/pSK, PCV2 (ORF3)-EGFP/pSK, PCV2 (ORF4)-EGFP/pSK, and PCV2(ORF5)-EGFP/pSK showed no fluorescent signals in transfected cells, while green fluorescent signals were observed in the nucleus of PK-15 cells after PCV2 (ORF2)-EGFP/pSK transfection. The presence of ORF2-EGFP fusion protein was demonstrated by dual signals of green fluorescence and anti-PCV2 antibodies conjugated with rhodamine in IFA. Furthermore, the released EGFP-fused PCV2 genome was demonstrated by real-time PCR.
