Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG) induces Apoptosis and Cell Cycle Arrest in B16F10 Murine Melanoma Cells

Rozanaliza RADZI; Tomohiro OSAKI; Takeshi TSUKA; Tomohiro IMAGAWA; Saburo MINAMI; Yuji NAKAYAMA; Yoshiharu OKAMOTO

doi:10.1292/jvms.11-0464

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Photodynamic Hyperthermal Therapy with Indocyanine Green (ICG) induces Apoptosis and Cell Cycle Arrest in B16F10 Murine Melanoma Cells

Rozanaliza RADZI, Tomohiro OSAKI, Takeshi TSUKA, Tomohiro IMAGAWA, Saburo MINAMI, Yuji NAKAYAMA, Yoshiharu OKAMOTO

著者情報

キーワード: apoptosis, cell cycle arrest, indocyanine green, photodynamic hyperthermal therapy

ジャーナルフリー早期公開

論文ID: 11-0464

DOI https://doi.org/10.1292/jvms.11-0464

この記事には本公開記事があります。

J-STAGE : jvms 74 5 pp.545-551

詳細

抄録

We examined the effects of photodynamic hyperthemal therapy (PHT), which is a combination of photodynamic therapy (PDT) and hyperthermia (HT), on the apoptosis and cell cycle progression of murine melanoma B16F10 cells. The percentage of apoptotic cell was determined by flow cytometry using fluorescein isothiocyanate (FITC)-conjugated Annexin V and propidium iodide (PI) double staining. The cell cycle analysis was performed by PI staining with flow cytometry. The expression of cyclins and heat shock protein 70 (Hsp70) were examined by a Western blotting analysis. PHT induces death in B16F10 cells, and PHT-mediated apoptosis occurred acutely and persistently in vitro. Our study demonstrated that PHT using indocyanine green (ICG) and near infrared (NIR) light source induces apoptosis and G0/G1 cell cycle arrest in the B16F10 cells.

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