Effects of Storage Temperature and Semen Extender on Stored Canine Semen

Abstract

The objective of the present study was to determine an optimum temperature and extender for short-term transport of canine ejaculated semen. There was no significant difference on the qualities of semen diluted two kinds of extender, egg yolk Tris-citrate fructose (EYT-FC) or glucose (EYT-GC) extender between the 2°C or 8°C or 12°C and 4°C control groups during storage for up to 48 hr, while the 16–24°C groups showed decreased sperm motility during storage for 48 hr. However, 2°C group showed slightly lower sperm motility and slightly higher sperm abnormality than 4°C group. Therefore, we concluded that semen qualities can be maintained for up to 48 hr when canine semen samples are extended with EYT-FC or EYT-GC and stored at the range of 4–12°C.