Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene

Masashi WATANABE; Kazuaki TANAKA; Tatsuya TAKIZAWA; Kazuhito SEGAWA; Sakurako NEO; Ryo TSUCHIYA; Michiko MURATA; Masaru MURAKAMI; Masaharu HISASUE

doi:10.1292/jvms.13-0316

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Characterization of a Canine Tetranucleotide Microsatellite Marker Located in the First Intron of the Tumor Necrosis Factor Alpha Gene

Masashi WATANABE, Kazuaki TANAKA, Tatsuya TAKIZAWA, Kazuhito SEGAWA, Sakurako NEO, Ryo TSUCHIYA, Michiko MURATA, Masaru MURAKAMI, Masaharu HISASUE

著者情報

Masashi WATANABE
Laboratory of Veterinary Internal Medicine II, Faculty of Veterinary Medicine, Azabu University
Kazuaki TANAKA
Laboratory of Animal Biotechnology, Faculty of Veterinary Medicine, Azabu University
Tatsuya TAKIZAWA
Laboratory of Animal Biotechnology, Faculty of Veterinary Medicine, Azabu University
Kazuhito SEGAWA
Laboratory of Veterinary Internal Medicine II, Faculty of Veterinary Medicine, Azabu University
Sakurako NEO
Laboratory of Veterinary Internal Medicine II, Faculty of Veterinary Medicine, Azabu University
Ryo TSUCHIYA
Laboratory of Veterinary Internal Medicine II, Faculty of Veterinary Medicine, Azabu University
Michiko MURATA
Laboratory of Molecular Biology, Faculty of Veterinary Medicine, Azabu University
Masaru MURAKAMI
Laboratory of Molecular Biology, Faculty of Veterinary Medicine, Azabu University
Masaharu HISASUE
Laboratory of Veterinary Internal Medicine II, Faculty of Veterinary Medicine, Azabu University

キーワード: canineTNFAgene, tetranucleotide microsatellite

ジャーナルフリー早期公開

論文ID: 13-0316

DOI https://doi.org/10.1292/jvms.13-0316

この記事には本公開記事があります。

The final version of this article is now available: Vol. 76 (2014), No. 1 pp. 119-122

詳細

抄録

A polymorphic tetranucleotide (GAAT)_n microsatellite in the first intron of the canine tumor necrosis factor alpha (TNFA) gene was characterized in this study; 139 dogs were analyzed: 22 Beagles, 26 Chihuahuas, 20 Miniature Dachshunds, 24 Miniature Poodles, 22 Pembroke Welsh Corgis and 25 Shiba Inus. We detected the presence of the 4 alleles (GAAT)₅, (GAAT)₆, (GAAT)₇ and (GAAT)₈, including 9 of the 10 expected genotypes. The expected heterozygosity (He) and the polymorphic information content (PIC) value of this microsatellite locus varied from 0.389 to 0.749 and from 0.333 to 0.682, respectively, among the 6 breeds. The allelic frequency differed greatly among breeds, but this microsatellite marker was highly polymorphic and could be a useful marker for the canine TNFA gene.

責任著者(Corresponding author)

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