Use of the MALDI BioTyper system and rapid BACpro with MALDI-TOF MS for rapid identification of microorganisms causing bacterial urinary tract infection in feline urine samples

Hiroto MAEDA; Kaori HAYASHI; Takayuki ISHIGE; Tomohiro SUNAGAWA; Shigeko TANIGAWA; Mika MISHINA; Toshifumi WATANABE; Kazuyuki SOGAWA

doi:10.1292/jvms.18-0145

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Use of the MALDI BioTyper system and rapid BACpro with MALDI-TOF MS for rapid identification of microorganisms causing bacterial urinary tract infection in feline urine samples

Hiroto MAEDA, Kaori HAYASHI, Takayuki ISHIGE, Tomohiro SUNAGAWA, Shigeko TANIGAWA, Mika MISHINA, Toshifumi WATANABE, Kazuyuki SOGAWA

著者情報

キーワード: cat, lower urinary tract disease, matrix-assisted laser desorption ionization-time of flight mass spectrometry, urine

ジャーナルフリー早期公開

論文ID: 18-0145

DOI https://doi.org/10.1292/jvms.18-0145

この記事には本公開記事があります。

The final version of this article is now available: Vol. 80 (2018), No. 10 pp. 1490-1494

詳細

抄録

With the increasing number of cats kept as pets, opportunities to treat cats with lower urinary tract disease (LUTD) have recently increased in the clinical veterinary field. Urine samples collected from 50 cats with bacterial cystitis brought to Maeda Veterinary Hospital between August 10, 2015 and March 31, 2017 were used in the study. Sample preparation of the urine was performed using a MALDI Sepsityper kit and rapid BACpro. To identify the isolates, MALDI-TOF MS was performed on an AutoFlex TOF/TOF mass spectrometer. MALDI-TOF MS using rapid BACpro for pretreatment was found to be a quick and reliable method for identification of bacteria from infected urine, with a shortened analysis time enabling earlier and more accurate selection of antibiotics for treatment of feline LUTD.

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