Acetyl Salicylic Acid Alleviates Increased Susceptibility to Thrombosis in Pial Microvessels of Dehydrated Mice

Abstract

Effect of water deprivation for 1, 2 and 3 days, on blood parameters and photochemically-induced thrombosis in pial microvessels of the mouse were investigated. Blood data of dehydrated mice confirmed hemoconcentration with significant increases in urea, total proteins and cholesterol. The time required for the first platelet aggregate to appear and time to full occlusion in pial arterioles were significantly shorter in dehydrated mice than in control, thus suggesting an increased susceptibility to cerebrovascular thrombosis. The effect of acetyl salicylic acid (ASA), 100 mg/kg (i.p.), in alleviating the accelerated thrombosis in dehydrated mice was also investigated. Mice were deprived of drinking water for a 24-hr period prior to the injection of ASA and the photochemical aggregation procedure. Acetyl salicylic acid significantly prolonged both time to platelet aggregation and to full occlusion of the vessel in dehydrated mice, thus indicating a protective effect.