Enhancement of mRNA Synthesis from Marek's Disease Virus Genome in the Lymphoblastoid Cell Line, MDCC-MSB1, by 5-Azacytidine

Masanobu HAYASHI; Tatsuya FURUICHI; Shaomin REN; Emiko ISOGAI; Meihan NONOYAMA; Shigeo NAMIOKA

doi:10.1292/jvms.56.287

Masanobu HAYASHI, Tatsuya FURUICHI, Shaomin REN, Emiko ISOGAI, Meihan NONOYAMA, Shigeo NAMIOKA

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Keywords: lymphoblastoid cell, MDV DNA, methylation, mRNA synthesis, 5-AzC

JOURNAL FREE ACCESS

1994 Volume 56 Issue 2 Pages 287-291

DOI https://doi.org/10.1292/jvms.56.287

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Abstract

Marek's disease virus (MDV) DNA in latently infected lymphoblastoid cell lines is considerably methylated. A treatment of the MDV-derived lymphoblastoid cell line, MDCC-MSB1 (MSB1), with 5-azacytidine (5-AzC) resulted in a hypomethylation of MDV DNA and an increase in mRNA from certain portions of the MDV DNA. These results suggest methylation of MDV DNA as being one of the factors associated with a repression of transcription of MDV DNA in the lymphoblastoid cell line, MSB1.

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