Identification of Bovine Serum Transferrin Phenotypes by Polyacrylamide Gel Isoelectric Focusing (PAGIEF)

Abstract

Each of four common homozygous bovine serum transferrins Tf A, Tf D₁, Tf D₂, and Tf E gave only two main bands in polyacrylamide gel isoelectric focusing (PAGIEF). This is considered to be due to the co-migration of the main components 2a and 3a with the main components 2b and 3b, respectively, in PAGIEF. Ten phenotypes, which are controlled by four alleles Tf^A, Tf^D1, Tf_SUPD2, and Tf^E, were distinguishable from each other in PAGIEF.