Immunoblotting Analysis of Somatic Components of Dirofilaria immitis

Abstract

SDS-PAGE analysis of Dirofilaria immitis extracts demonstrated the complexicity of protein components of microfilariae, similar to that of adult male and female worms. Immunoblotting analysis using sera from microfilaremic and amicrofilaremic dogs with D. Immitis infection suggest that antigenic components in the low molecular weight region may be related to the anti-microfilarial mechanism of the host.