Hantaan Virus Persistently Infects Marmoset B-Lymphoblastoid Cell Line

Abstract

To investigate lymphoid cell Hantavirus (HV) interaction, we examined the replication of the HV strains, Hantaan 76-118(HTN), SR-11, and B-1 with the B95d; LYM-1, CGM1, JM, Jurkat, MOLT-4, AT(L)5KY, BW5147, L1210, and Vero/E6 cells. HV-growth was determined by immunofluorescence and focus-forming assays. LYM-1 and Vero/E6 supported the growth of all HV strains, and B95a only HTN, while remaining cells did not sustain these strains. This is the first report to indicate the HV-replication in lymphoid cell lines. B95a and LYM-1 cells may be useful for revealing the mechanism of HV-infection in animals.