Detection of Mycoplasma DNA in Veterinary Live Virus Vaccines by the Polymerase Chain Reaction

Akemi KOJIMA; Toshio TAKAHASHI; Mayumi KIJIMA; Yasuaki OGIKUBO; Yutaka TAMURA; Ryo HARASAWA

doi:10.1292/jvms.58.10_1045

Journal of Veterinary Medical Science

Online ISSN : 1347-7439
Print ISSN : 0916-7250
ISSN-L : 0916-7250

Detection of Mycoplasma DNA in Veterinary Live Virus Vaccines by the Polymerase Chain Reaction

Akemi KOJIMA, Toshio TAKAHASHI, Mayumi KIJIMA, Yasuaki OGIKUBO, Yutaka TAMURA, Ryo HARASAWA

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Keywords: mycoplasma contamination, polymerase chain reaction, vaccine

JOURNAL FREE ACCESS

1996 Volume 58 Issue 10 Pages 1045-1048

DOI https://doi.org/10.1292/jvms.58.10_1045

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Abstract

We examined bovine, porcine, canine, feline, avian and mink veterinary live vaccines for the presence of mycoplasma DNA by the polymerase chain reaction including nested-pair primers. Mycoplasma DNA was detected in 22 of the 61 commercial veterinary vaccines (36.1%), although these vaccines did not contain any viable mycoplasma cells.

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