Surfactant-coated lipase was prepared with a newly synthesized surfactant. The synthesis reaction of glycerides was investigated using the lipase and substrates of glycerol and oleic acid. The activity of the enzyme, which is significantly higher than that of native enzyme, depends strongly on the water content, and the optimal water content is about 3040 mol%. The main product of this enzyme reaction was monoolein under the present experimental conditions.
