口腔レンサ球菌とブドウ球菌の相互作用に関する研究特に緑色レンサ球菌のブドウ球菌に対する発育阻止作用について

抄録

The inhibitory action of Streptococcus (Str.) sanguis I 10556, Str. sanguis II 10557, Str. salivarius I 9759, Str. salivarius II 13419, Str. mitis 9811 and Str. MG 9895 on the growth of Staphylococcus (Staph.) aureus 209 P and 48 strains of Staph. aureus isolated from the saliva was investigated using the spread and the stab method.
The growth of Staph. aureus was inhibited by 98% by Str. sanguis I, II and by 62% by Str. mitis but not inhibited by Str. salivarius I, II and Str. MG. On ß hemolytic streptococci (Group G and H), only some of above mentioned species (Str. sanguis II, Str. salivarius I and II) have shown inhibitory activity.
It was found that 61% of laboratory-stocked 33 strains of viridans streptococci and 71% of newly isolated 45 strains inhibited the growth of before mentioned Staph. aureus. The newly isolated ones showed higher incidence of the inhibitory activity than laboratory-stocked ones. However, it can not be taken as it was, because some strains were observed to lose or increase their inhibitory activity during the stock culture.
In four persons, streptococci and staphylococci were sought in various sites of the oral cavity. Then, the interactions between them were investigated. On the one hand, viridans streptococci were isolated in high frequency from various sites and staphylococci were found only from salivaor tongue and not from dental plaque at all, on the other. Inhibitory action of streptococci strains from dental plaque and tongue was found stronger than those from saliva.
The inhibitory activities were found strongest in neutral, media and were passablethrough the dialysis membranes and completely destroyed by heating at 60°C for 30 minutes.