秋田県におけるImpatien snecrotic spot virus (INSV) の発生

抄録

Flower crops lisianthus, impatiens, New Guinea impatiens, and cyclamen showing necrosis or necrotic ring spot symptoms were collected in 1999 in Akita Prefecture. These isolates did not react positively to DIBA with polyclonal antibody against TSWV, but reacted similarly to tospovirus in sap inoculation test. RT-PCR was conducted to detect Tomato spotted wilt virus (TSWV) and Impatiens necrotic spot virus (INSV) using degenerate for TSWV and INSV, and INSV specific primers, and a product of the predicted size was obtained with both primers. To characterize this INSV isolate obtained from lisianthus, we determined the N protein sequence. Sequence comparison of the N protein showed a 98.9% identity in the amino acid with INSV NL-07. This is the first report of INSV occurrence in Akita Prefecture.