抄録
1. The genetic association between prophage P22 (iota) and Salmonella genome can be demonstrated by bacterial crosses. The donor strain used is a P22 (iota) lysogenic Salmonella paratyphi B 6617 (O-1, 4, 5, 12) and the recipient is a P22 (iota) nonlysogenic his+, pro+ recombinant obtained from a cross between S. emek (O-(8), 20) donor and S. paratyphi B var. odense (O-1, 4, 12) recipient or a his+ recombinant (O-(8)) from a cross between S.emek (O-(8), 20) donor and S. paratyphi B 8006 (O-4, 5, 12) recipient. In the cross, some of the recombinants selected for possession of the pro+ locus of the donor have P22 (iota).
In the reversed cross, with a nonlysogenic male strain S. emek (O-(8), 20) to a lysogenic female strain recombinant (O-(8)) from a cross between S. emek (O-(8), 20) donor and S. paratyphi B var, odense (O-1, 4, 12) recipient, some of the recombinants selected for possession of the pro+ or nic+ locus of the donor are transferred the P22 (iota) nonlysogeny of the donor. These results indicate that the genetic determinant of P22 (iota) lysogeny maps near pro not so far from nic.
2. In a cross of a P22 (iota) nonlysogenic S. emek (O-(8), 20) F' donor with a P22 (iota) lysogenic O-(8) his+ recombinant F-recipient from a cross between S. emek (O-(8), 20) F' donor and S.paratyphi B var. odense (O-1, 4, 12) recipient, O-antigens of some of the pro+ or nic+ recombinants are changed from O-(8) to O-(8), 20. From a point of frequency of O antigen transfer, the genetic determinant of O antigen 20 is found to be situated near nic, not far away from pro.
3. There are no manifestation of O antigen 20 in semirough recombinants having O antigen 4, 12 from a cross between a donor of S. abony (O-1, 4, 5, 12) Hfr and a recipient of S. emek (O-(8), 20) F-. The donor is the O-(8) his+ recombinant from a cross of a S. emek (O-(8), 20) F' donor to a S.paratyphi B 8006 (O-4, 5, 12) F- recipient and the recipient is semirough recombinant having O antigen 4, 12. In this cross, all of the recombinants which receive O antigen (8) from the donor become to manifest O antigen 20. This result indicates that O antigen (8) is necessary for the manifestation of the O antigen 20.
