抄録
The interaction of gangliosides, protein and of Ca2+ was studies, for clarifying the molecular mechanism of excitation. Purified gangliosides, which were used in this studies were separated on silicic acid column by the stepwise elution of chloroform-methanol-4N NH4 OH mixture. The interaction between gangliosides and protein was studied in the biphasic solvent system consisting with chloroform, methanol and water.
The results are summarised as follows :
1) All of the added GT and GD was recovered from the upperphase even under the presence of Ca2+. On the other hand, about 20% of added GM was recovered in the lower phase.
2) Ganglioside-BSA-Ca2+ complex was formed at the interface by the addition of Ca2+ and BSA. Maximal complex formation was observed, when 3mg of BSA and 10 μmmoles of CaCl2 were added to 0.5 μmole of ganglioside.
3) Complex forming ability of each ganglioside was the following order; GT>GD>GM.
4) By the addition of methylated albumin, as the model basic protein, ganglioside-protein complex was formed at the interface in the absene of cation.
5) Two types of complex, which were described above, were dissolved by the addition of K+, NH4+ or acetylcholine.
6) Interelationship between the molecular mechanism of excitation of synaptic membrane, which contains large amount of gangliosides, and gangliosides was disscussed.
