Abstract
RNase Tl oligonucleotide mapping analysis using 32P contributes to the identification and classification of various kinds of viruses. The MI-320 strain of bovine enterovirus was labelled with H3PO4 in vivo for the mapping analysis. One cycle of CsCl centrifugation of the crude virus derived from 4 Roux cultures yielded a significant amout of purified virion from which the virion RNA was isolated. Agarose-acrylamide gel electrophoresis of this RNA gave a single sharp peak at a position slightly ahead of the 28 S mouse ribosomal RNA. The specific radioactivity of this virion RNA was not sufficient for the RNase Tl oligonucleotide mapping analysis.
