抄録
In the absence of intracellular enzymes the extracellular enzymes continue to produce 2, 3 butanediol up to a certain period but gradually the quantity of acetoin increases and that of butanediol decreases. The decreases of 2, 3 butane-diol and the simultaneous increase in the quantity of acetoin is rapid in the normal culture, which contains extra and intracellular enzymes. The formation of acid in the mixture is rapid in the normal culture, whereas if only the extra-cellular enzymes are present, the formation of acid is slow. In the presence of intracellular enzymes the mixture is alkaline.
The enzymes present in the bacterial cell are capable of utilising sugar even after the death of the organism, but acetoin and butanediol are not formed in the mixture.
