Cell micropatterning techniques have greatly contributed to fundamental studies of cell biology. However, they have nly limited application to regenerative medicine so far. One of the most significant challenges in the field of regenerative medicine is introducing blood vascular systems into artificial tissue constructs. Recently, we have developed the technique called cell transfer printing, which has the potential to solve the issue. In this paper, we describe the preparation of a micropatterned substrate by photocatalytic lithography and its surface characterization, and then provide a brief overview of transfer printing of micropatterned endothelial cells. Possible mechanisms of cell transfer printing and its further prospects are also discussed.