COMPARISION OF SBT SYSTEMS BETWEEN GENETIC KIT HLA SEQUENCING ASSAY (VISIBLE) AND SEQUENCING BASED TYPING KIT (APPLIED BIOSYSTEMS) METHODS.

抄録

The SBT (sequencing based typing) system has many advantages among HLA genotyping methods which have been developed f∞oor high resolution typing. At present, several allele detection systems for SBT were developed. Two SBT methods, Genetic Kit HLA sequencing Assay (VISIBLE) and Sequencing-Based Kit (Applied Biosystems) are being used as routine methods in our laboratory. In this study, in order to evaluate them as reliable SBT technique systems, we have compared accuracy and typing results between them. As a result, typing results of both techniques were in agreement with to each other, revealing that both methods were suitable for HLA high resolution typing due to their reliability and high accuracy.