LC-MS/LC-UV analysis of type-A and -B trichothecenes after multifunctional MycoSep clean-up
2003 年 2003 巻 Suppl3 号 p. 225-232
詳細
2003 年 2003 巻 Suppl3 号 p. 225-232
A quick and efficient method for the determination of type-A and -B trichothecenes is described employing high performance liquid chromatography with UV (ultra violet) and MS (mass spectrometry) detection. Using Mycosep multifunctional clean-up columns multi-toxin clean-up was done within one procedure and one solvent system, thus saving considerable time in comparison with other clean-up methods. The method was tested and validated in corn, wheat and barley with MS detection for the most common A-Trichothecenes (T-2 toxin, HT-2 toxin and DAS), using T-2 toxin-d3 as internal standard. For most prevalent B-Trichothecenes, i.e. deoxynivalenol (DON), nivalenol (NIV), 3-acetyl-deoxynivalenol, and fusarenon X (FUS-X), the method was validated with UV absorption and calibrated for MS detection.
