A practical method for measuring deoxynivalenol, nivalenol and T-2+HT-2 toxin in foods by an enzyme-linked immunosorbent assay using monoclonal antibodies

抄録

Trichothecene mycotoxins such as deoxynivalenol (DON), nivalenol (NIV), and T-2 toxin (T-2) are representative mycotoxins that contaminate major cereal crops. Methods such as gas chromatography-mass spectrometry (GC-MS), liquid chromatography-mass spectrometry (LC-MS), and high performance liquid chromatography (HPLC) are generally used to measure these toxins. These methods are considered unsuitable for routine use when many samples must be treated within a short time. Enzyme-linked immunosorbent assay (ELISA) would be equivalent to a method of measurement at high throughput, such as screening. An antibody that distinguishes NIV from DON was required to precisely measure the above three kinds of toxins by an ELISA method. We were successful in developing a monoclonal antibody for 3, 4, 15-triacetyl-NIV, which was generated under conditions where an acetyl group was introduced at the C-4 position of NIV without an acetylation at the C-7 position. Simultaneously, we obtained a monoclonal antibody for both 3, 4, 15-triacetyl-NIV and 3, 15-diacetyl-DON as well as one for acetyl-T-2. Three kinds of ELISA kits for the individual measurement of DON, NIV, and T-2+HT-2 were constructed using these monoclonal antibodies. The components derived from wheat extracts did not influence our kits. An investigation of the performance of the kits revealed high reproducibility and a good correlation with GC-MS. Thus, we could offer simpler and more useful ELISA systems as alternative methods to GC-MS, LC-MS, and HPLC for screening contaminated cereals and foods.