Validation of an ELISA test kit for the detection of deoxynivalenol in several food commodities
2003 年 2003 巻 Suppl3 号 p. 295-302
詳細
2003 年 2003 巻 Suppl3 号 p. 295-302
An ELISA Microtiter Plate, Deoxynivalenol (DON) Test called AgraQuant® was validated to measure DON in a range from 0.25-5.0mg/kg. The test is performed as a solid phase direct competitive ELISA using a horseradish peroxidase conjugate as the competing, measurable entity. For the test method, DON is extracted from ground sample with distilled water and diluted sample extract plus conjugate are mixed and then added to the antibody-coated microwells. After 15 minutes incubation at room temperature, the plate is washed and substrate is added and allowed to incubate for an additional 5 minutes. Stop solution is then added and the intensity of the resulting yellow color is measured optically with a microplate reader at 450 nm. Results obtained from internal validation studies assessing accelerated stability, accuracy, precision and limit of detection in wheat and other grains and grain products, comparison of method to HPLC and ruggedness of the test kits determined this test to be rugged, sensitive, accurate, precise and effective comparable to HPLC for measuring DON ranging from 0.25-5.0mg/kg in several commodities.
