Analysis of trichothecenes in beer and barley tea by LC/MS/MS
2003 年 2003 巻 Suppl3 号 p. 303-308
詳細
2003 年 2003 巻 Suppl3 号 p. 303-308
A simple method for the analysis of trichothecenes [Type A: diacetoxyscirpenol (DAS), neosolaniol (NEO), HT-2 toxin (HT-2), and T-2 toxin (T-2), Type B: deoxynivalenol (DON), nivalenol (NIV), fusarenon-X (F-X)] using liquid chromatography electrospray ionization tandem mass spectrometry (LC/MS/MS) was developed. Trichothecenes was extracted with ethyl acetate/methanol and diluted with water/methanol for injection into the LC/MS/MS. The LC separation was performed with an octadecylated silica column (3μm particles, 150 mm length, 2 mm i.d.) with a flow-rate of 0.2 mL/min, using a mobile phase consisting of water, methanol, and acetonitrile. MS/MS was used in multiple reaction monitoring, employing electrospray ionization (ESI-MRM). The recoveries of trichothecenes from beer and barley tea at 1μg/L (Type A), 10μg/L (Type B) were 68.1-127.5 % (beer), 52.5-115.2 % (barley tea). The lower limits of quantification were 0.07-1.0μg/L and 0.01-0.8μg/L. The method was applied to ten domestic beers and five commercial barley teas. No trichothecenes was detectable. This method may have applications in quality assurance.
