New rapid clean-up device for ochratoxin A
キーワード:
ochratoxin A,
sample clean-up,
high performance liquid chromatography,
food analysis,
Mycosep®
2003 年 2003 巻 Suppl3 号 p. 315-318
詳細
2003 年 2003 巻 Suppl3 号 p. 315-318
High Performance Liquid Chromatography (HPLC) with fluorescence detection (FLD) and Thin Layer Chromatography (TLC) are widely used analytical methods for the determination of Ochratoxin A (OTA). As normally the crude extract cannot be used directly for these analyses, a clean-up step is necessary. Commonly used clean-up procedures comprise immunoaffinity columns (IAC), liquid liquid extraction (LLE) and solid phase extraction (SPE). The performance of a new single step clean-up column Mycosep® 229 Ochra from Romerlabs® was tested in matrices often contaminated with OTA like green coffee beans, raisins and various kinds of cereals. In all these matrices an average recovery of more than 90% (range 2.6-92μg/kg; n=24) of the added OTA could be obtained. Additionally, a certified reference material (CRM), OTA in wheat, was tested with the column. An average recovery of 96.2% (n=9) was achieved for the CRM proving the applicability and high accuracy of the developed method.
