Comparison of ELISA-based screening and minicolumn test kits with liquid chromatography for the determination ofaflatoxins in corn

抄録

Two enzyme-linked immunosorbent assay (ELISA)-based screening and a developed minicolumn test kits were compared with a quantitative method for aflatoxin determination in corn. The samples analyzed included one set of artificially-contaminated corn containing different aflatoxin levels (0, 10, 20 and 50μg aflatoxin B₁/kg sample), and a set of naturally-contaminated corn samples containing the same levels of aflatoxin contamination. Data of the analysis with each test kit were evaluated and compared with the HPLC data of the test samples. Results showed that both the two ELISA-based test kits gave positive responses of 71 % and the “lahar” minicolumn test kit, 83.3 %. The performance of the locally-developed aflatoxin test kit which utilizes a “lahar” minicolumn is comparable to that of the commercial test kits.