Gas Chromatography (ECD-GC)によるGriseofulvinの定量

抄録

Griseofulvin could be assayed by gas chromatographic method. A Shimadzu GC-3AE instrument equipped with an electron-capture detector of pulse type and 2,000×4mm of glass column packed with 1.5% SE-30 on Chromosorb W (60-80 mesh) was employed. In the refining process of griseofulvin from extract, chromatography on 70×6mm Florisil colum was applied. Serum griseofulvin levels in 5 rabbits given simgle oral doses of 30mg/kg showed maximum concentration of 0.57∼1.50 μg/ml at 8 or 12 hours after administration, and decreased to 0.15∼0.30 μg/ml at 24 hours. Skin griseofulvin levels in the same rabbits showed maximum concentration at 4 hours in one case and at 8 or 12 hours in other cases, and it was 0.38∼0.83 μg/g. They decreased 0.07∼0.18 μg/g at 24 hours in all cases. In rabbits, skin griseofulvin levels paralleled serum griseofulvin levels and the former was lower than the later for the most part. Serum griseofulvin levels in 5 human subjects given single oral doses of 500mg showed maximum concentraton of 0.30∼2.30 μg/ml at 4 or 8 hours and decreased to 0.30∼1.04 μg/ml at 24 hours.