Ultrastructural Immunocytochemistry of Tetanus Toxin Binding Sites to Spinal Ganglia of Chick Embryo in Tissue Culture

Abstract

By the use of immunoperoxidase technique, tetanus toxin binding sites were ultrastructurally demonstrated in monolayer culture after three days in cultures derived from the spinal ganglia of the 10 daychick embryo. The toxin binding sites, seen as electron dense reaction products, were clearly limited to the surface membranes of the neuronal processes, but not of the nerve cell bodies.