Abstract
We previously demonstrated that Hosobawadan was an effective anti-oxidative vegetable that contained anti-oxidative constituents such as D(+)-chicoric acid (ChA), chlorogenic acid (ChgA), caffeic acid (CA), luteolin 7-O-β-D-glucuronide (LU-gluc) and luteolin 7-O-β-D-glucopyranoside (LU-glc). In the present study, we first examined anti-LDL oxidative activity of Hosobawadan and found that the strength of anti-oxidative activity for LDL was dependent upon ChA, LU-gluc and LU-glc. This was due to ChA, which exhibited moderate activity and was abundant in Hosobawadan, with LU-gluc and LU-glc also exhibiting strong activity. We then examined the levels of these polyphenol compounds in rat plasma after oral administration of Hosobawadan extract (ChA 200mg/kg body weight). ChA was detected at the highest concentrations (2.9±1.6μg/mL) in the rat plasma 15min after oral administration. Conversely, luteolin (LU) was detected in rat plasma treated with glucuronidase/sulfatase, suggesting that LU in plasma existed as LU conjugates.
