Abstract
The mean body weight of male mice fed a high-fat diet and seaberry leaf polyphenols (SBLPP) juice for eight weeks was significantly lower than that of controls, with the weights of abdominal adipose tissue and liver being particularly low. Additionally, blood GTP activity in experimental mice was significantly lower than in controls, demonstrating the inhibitory effects of SBLPP juice on development of fatty liver, which may otherwise be induced by a high-fat diet. Furthermore, expression of acyl-CoA oxidase (ACO), medium-chain acyl-CoA dehydrogenase (MCAD), and fatty acid beta-oxidization enzymes significantly increased in experimental mice. In vitro experiments showed that SBLPP juice suppressed pancreatic lipase activity in a concentration-dependent manner, with IC50 being 4.5ppm. It was therefore suggested that, in mice fed a high-fat and SBLPP juice diet, gene expression of fatty acid beta-oxidation enzymes in the liver was enhanced, which increased the lipid metabolism and inhibited the pancreatic lipase activity, thus reducing lipid transportation to the body and increasing fecal lipid excretion. As a result, body fat deposition was inhibited.
