Effect of Burdock and Cabbage Powder Administration on the Excretion of Trp-P1 and its Metabolite in Rats

Abstract

Trp-P1 is a known carcinogen in rats and humans and is produced during cooking fish or meat. To investigate the effect of burdock and cabbage powder administration on the excretion of Trp-P1 and its metabolite, n-OH Trp-P1, rats were fed a diet containing 10% burdock or cabbage powder, followed by peroral administration of Trp-P1 (750μg) for 4 days. HPLC and the bacterial mutagenicity assay analyses of the feces and urine of Trp-P1-fed rats found mutagenic zones corresponding to Trp-P1 and its metabolite at Rf 0.1 and 0.3, respectively, on the thin layer chromatography C18 gel (Trp-P1, UV absorbance ; n-OH Trp-P1, electrochemical detector absorbance). Moreover, the metabolite was detected in the feces, but not the urine. Following administration of burdock and cabbage powder, urinary Trp-P1 excretion significantly decreased from 31.9±9.1μg to 25.2±4.7 and 14.3±2.6μg, respectively (p<0.05), while fecal metabolite excretion showed no significant difference.