Abstract
Influence of culture medium on the composition of water-soluble polysaccharides in the Ganoderma lucidum fruit body was investigated using various kinds of pruned off branches as the medium. Total carbohydrate content of water-soluble polysaccharides obtained from the fruit body differed among them : persimmon (90.2mg/10g dry weight of fruit body), mulberry (49.8mg), Japanese cherry (33.1mg), Japanese plum (34.1mg), apple (52.1mg), Japanese oak (28.3mg), and grape (28.4mg). Neutral sugar composition and neutral-sugar linkage analyses indicated that galactan-like polysaccharide and xylan-like polysaccharide, in addition to β-glucan, were present at different ratios in the water-soluble polysaccharide fractions. This means that the content of these polysaccharides in the fruit bodies is influenced by the culture medium, i.e., the kind of pruned off branch.