New Tannins Contained in Cassia mimosoides L. var. nomame Makino and Their Lipase Inhibitory Activity

Abstract

In order to isolate ingredients with lipase inhibitory activity in the ethanol extract of Cassia mimosoides L. var. nomame Makino, fractions were collected using liquid column chromatography and hig-performance liquid chromatography. This resulted in the isolation of ingredients I and II. Structural analysis showed that ingredient I was a known condensed tannin dimer. In contrast, ingredient II was found to be a novel condensed tannin trimer, but its final structure and stereostructure could not be successfully determined. Measurement of lipase inhibition activities showed that ingredients I and II had 50% inhibitory concentrations of 10 μg/mL and 0.8 μg/mL, respectively. Thus, it was confirmed that ingredient II had strong lipase inhibitory activity.