Validation of the quantification of phosphatidylserine in food supplements using quantitative phosphorus NMR

Abstract

Phosphatidylserine (PS) is a phospholipid that is common in the brain and nerve tissue, and is believed to improve cranial nerve function. Function claims related to memory maintenance have thus been accepted as "Food with Function Claims" in Japan, and they are mostly used for health supplements. We have recently reported the applicability of phosphorus-31 (³¹P) NMR for the quantification of phospholipids despite the lack of validation data. In the present study, we focused on the method of modification and the validation of quantifying PS in health supplements using ³¹P-NMR. The obtained results on the validation parameters satisfied the global standards set by Codex and AOAC International.