Abstract
The 2, 4-dinitrophenylhydrazine (DNP) method has been widely used for the determination of ascorbic acid, however, this method is complicated and time consuming, and is influenced by the presence of various compounds, such as sugars (glucose, fructose and sucrose etc.) and keto acids. In the present study, dehydroascorbic acid osazone was separated from sugar osazones on TLC plate and determined by a TLC densitometer. This method was applied to the determination of ascorbic acid in some vegetables and fruits. In comparison with the conventional DNP method, the present method is simple and more rapid. Ascorbic acid samples can be measured both qualitatively and quantitatively with a comparatively high sensitivity (10-9 gram order) in the proposed procedure.
