Abstract
To obtain fundamental information on the production of fucose-containing xyloglucan oligosaccharides from okara by hydrolysis with industrial enzyme preparation, we have studied the structure of the oligosaccharide units of xyloglucan in okara hemicellulose, and hydrolysis mechanism of commercial enzyme preparation on okara hemicellulose. The oligosaccharide units of xyloglucan in okara hemicellulose, which was analyzed by enzymatic digestion (a xyloglucanase and an isoprimeverose-producing oligoxyloglucan hydrolase) followed by anion-exchange chromatography with pulsedamperometric detection, were XXXG, XXLG, XLXG, XXFG, XLLG, and XLFG [where each (1→4)-β-linked D-glucosyl residue in the backbone is given a one-letter code according to its substituents: G=β-D-Glc; X=α-D-Xyl-(1→6)-β-D-Glc; L=β-D-Glc-(1→2)-a-D-Xyl-(1→6)β-D-Glc; F=α-L-Fuc-(1→2)-β-D-Gal-(1→2)-α-D-Xyl-(1→6)-β-D-Glc] in an approximatemolar ratio of 33:2:4:27:3:31. An industrial cellulase preparation, Cellulosin T 2 was found to be an appropriate enzyme source for producing xyloglucan oligosaccharides from okarahemicellulose.
