Identification of Domestic Rice Cultivars by RAPD Method Using Milled Rice Grains as Samples

Abstract

As it is obliged that cultivars, location and year of production should be displayed on bag of milled rice grains, technology to identify them by the objective methods is indispensable. Milled rice grains produced at the several agricultural experiment stations were subjected to the RAPD method. Each DNA was extracted by CTAB method and amplified by PCR method using 10 mer primers. It became possible to identify the rice cultivars using milled rice grains as samples by the electrophore-sis of the PCR products among the dominant 10 rice cultivars in Japan.